This brings to nearly $500 million the amount the United States has pledged to the Syrian opposition since 2002, the State Department said.
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The new pledge was made by Deputy Secretary of State Antony Blinken at a regional security forum called the Manama Dialogue, in Bahrain.
"US assistance is helping keep schools open for Syrian children; restoring access to electricity and water infrastructure; supporting independent media and civil society to hold their local governments accountable, and building the capacity of the moderate opposition to play a role in a future Syria that respects human rights and the rule of law," the department said in a statement.
This assistance was announced a day after the United States revealed that is sending dozens of special ops forces to Syria to assist forces fighting the Islamic State group.