The Israeli army said it struck a Syrian military position on Thursday after presumed "errant fire" hit the Israeli-occupied sector of the Golan Heights.
A statement from the army said that "at least one projectile hit the northern Golan Heights."
A military spokeswoman told AFP it hit an open field without causing any damage or harm to anyone.
"Initial reviews suggest that this was a result of errant fire from the internal fighting in Syria," the statement said.
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"In response, the IDF (Israel Defence Forces) targeted a Syrian army position. Hits were confirmed."
Another army spokeswoman told AFP the forces had fired a Tamuz anti-tank missile at the Syrian military position.
Israel is closely monitoring the fighting between Syrian troops and Islamist rebels close to the armistice line on the Golan out of fear of a spillover of the internal conflict.
There has been repeated fire across the ceasefire line since the uprising in Syria erupted in March 2011, not all of it stray.
In June, Israeli warplanes attacked Syrian military headquarters and positions after an Israeli teenager was killed in what the Jewish state said was a cross-border attack by forces loyal to Syrian President Bashar al-Assad.
Israel seized 1,200 square kilometres (460 square miles) of the Golan Heights during the Six-Day War of 1967, then annexed it in 1981 in a move never recognised by the international community.