Islamist activists raided the last working bar in the central Tunisian town of Sidi Bouzid on Monday, smashing bottles and chasing away customers, witnesses said.
Around 50 activists burst into the bar in the Hotel Horchani in the centre of town, customers and staff told AFP.
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A young man who tried to film the raid was beaten by members of the group and taken to an unknown location, as angry hotel guests gathered at the scene.
The sale and consumption of alcohol is regulated but legal in Tunisia, traditionally a popular destination for tourists, especially from France.
Sidi Bouzid, the birthplace of the uprising that toppled veteran strongman Zine El Abidine Ben Ali last year, is a stronghold of the Salafist movement, which has grown increasingly assertive in recent months.
The North African country has witnessed numerous violent incidents linked to the hardliners, prompting opposition activists to accuse the Islamist-led coalition government of not doing enough to rein them in.